revised the manuscript. spHA1 extract analyses revealed anticancer potential. Many compounds have been identified Wogonoside including cyclo-(Leu-Leu), cyclo-(Pro-Phe), C17-sphinganine, hexanedioic acid, bis (2-ethylhexyl) ester, surfactin C14 and C15. The extract exhibited an IC50 of 68??1.8?g/mL and caused marked morphological changes in treated HepG2 cells. For mechanistic anticancer evaluation, 20 and 40?g/mL of bacterial extract were examined. The up-regulation of apoptosis-related genes’ expression, at mRNA and protein levels proved the involvement of P53-dependant mitochondrial apoptotic pathway. The anti-proliferative properties were confirmed by significant G2/M cell cycle arrest and PCNA down-regulation in the treated cells. Low cytotoxicity was observed in HPBL compared to HepG2 cells. In conclusion, results suggest that the apoptotic and anti-proliferative effects of spHA1 extract on HepG2 Wogonoside cells can provide it as a candidate for future pharmaceutical industries. strains from a marine origin may generate siderophores (low molecular weight Fe3+ chelating molecules) and loihichelins28. The progression of gastric adenocarcinoma cell lines (HM02), hepatocellular carcinoma (HepG2) and breast cancer (MCF-7) has been inhibited by apoptosis initiation and cell cycle arrest when treated with marine-derived sp(GWS-BW-H8hM strain)29,30. However, to the best of our knowledge, the novel strain has not been studied yet. Consequently, the characterization of bioactive compounds and evaluation of the possible anticancer potential of the bacterial extract against the HepG2 cell line were warranted. Results Identification of bacterial strain Morphological investigations showed two isolates of gram-negative short motile rods. Full-length 16S rRNA (about 1,500?bp) was sequenced and deposited under GenBank accession numbers (“type”:”entrez-nucleotide”,”attrs”:”text”:”KT223026″,”term_id”:”959360214″,”term_text”:”KT223026″KT223026) for HA1 isolate. The isolated HA1 16S GU2 rRNA sequence showed 99% similarity with the 16S rRNA gene sequence Wogonoside of BC7. However, the isolated strain is closer to evolutionary group (Fig.?1). Open in a separate window Figure 1 The phylogenetic tree based on 16?s rRNA sequences constructed by the neighbor-joining method, showing the position of strain HA1 and representatives of some related taxa. LCCMS-MS and NMR fraction analyses of H. HA1 extract The molecular networking of metabolome mass profile for the species sp.HA1 was screened and exhibited in 67 parent ions (nodes) (Fig.?2). Open in a separate window Figure 2 Molecular network of 67 parent ions produced from sp. HA1. The fuchsia nodes indicate to the whole molecular weights that have unique detected Wogonoside peaks in the molecular network. The green Nodes represent parent ions that are identified from the molecular networking database as they have been already isolated before. The yellow nodes indicate some identified compounds but with wrong precursor parent ions so they should not be recognized. The number of nodes was considered an indication of the unique peaks. Compounds with related molecular weight and shared class grouped together to form a cluster. Four clusters are noticed with some chemically related identified compounds. From the 67 nodes only 15 parent ions within the molecular network matched 15 known standards from the molecular networking database but three of them in the yellow nodes [M+H]+ (200.128, 228.164 and 393.265) have wrong Wogonoside precursor parent ions, so their identification cant be considered (Fig.?2 and Table ?Table11). Table 1 The parent and the fragments masses of the identified compounds compared with that of the standards from the molecular networking database. 288.218 [M+H]+ (Fig.?2 and Table ?Table1),1), was isolated from different fungus species31. C17-sphingolipid identified as mycotoxin (C17-SAMT) analog exerted potent toxicity in different assays32,33. The most potent identified compounds were two biosurfactants (Surfactin C14 and surfactin C15) with 1,022.48 and 1,036.52 [M+H]+ (Fig. S1). The parent ion masses fragmentation data for Surfactin C15 and.